A microfluidic system for dynamic yeast cell imaging.
نویسندگان
چکیده
The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.
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عنوان ژورنال:
- BioTechniques
دوره 44 1 شماره
صفحات -
تاریخ انتشار 2008